Description: Recent advances in genome editing tools using endonucleases such as TALENs, ZFNs, and CRISPRs, combined with genomic engineering technologies, have opened up a wide range of opportunities from applications in the basic sciences and disease biology research, to the potential for clinical applications and the development of new diagnostic tools. This complete guide to endonuclease-based genomic engineering gives readers a thorough understanding of this rapidly expanding field. Chapters cover the discovery, basic science, and application of these techniques, focusing particularly on their potential relevance to the treatment of cancer, and cardiovascular and immunological disease. The final section discusses the legal and ethical issues which accompany the technology. Providing authoritative coverage of the potential that genome editing and engineering have, this is an ideal reference for researchers and graduate students and those working in the biotechnology and pharmaceutical industries, as well as in a clinical setting.
Brief description: Krishnarao Appasani is the Founder and Chief Executive Officer of GeneExpression Systems. He is an award winning scientist, and has edited several books, including: Optogenetics: From neuronal function to mapping and disease biology (Cambridge, 2017); Genome-Wide Association Studies: From Polymorphism to Personalized Medicine (Cambridge, 2016); Epigenomics: From Chromatin Biology to Therapeutics (Cambridge, 2012); MicroRNAs: From Basic Science to Disease Biology (Cambridge, 2007); and RNA Interference: From Basic Science to Drug Development (Cambridge, 2005).
Review Quotes: 'This comprehensive book is welcome at this point of rapid growth in new technologies and discoveries such that keeping up with applications of genome engineering is challenging. Here we have both a general introduction to the field and to deep mechanisms and glimpses of future applications and ethical considerations - with gems for newcomers and experts alike.' George M. Church, Harvard University, Massachusetts and Massachusetts Institute of Technology