Description: This indispensable guide provides safety considerations as well as the basic technology for creating continuous cell lines from many diverse types of tissue and, by analogy, offers guidelines for extrapolation to other tissues not yet described. Includes specific procedures for the immortalization of kidney, and thyroid epithelia, hepatocytes, fibroblasts, chondrocytes, lymphocytes, astrocytes, and human umbilical vein endothelium. All methods contain step-by-step protocols, detailed instructions for reagent preparation and information on the latest specialized equipment and supplies.
