Description:
Fundamentals of Recombinant Protein Production, Purification and Characterization is organized into nine chapters in a logical fashion that cover an introduction to recombinant proteins and expression in different host expression systems, extraction, purification and analysis of proteins. This important reference features protocols, along with the advantages and disadvantage of each expression hosts and characterization technique (presented in tabular format) and offers detailed coverage of all aspects of protein production and processing (upstream and downstream processing) in one place. Finally, the book ends with different characterization techniques. Production of recombinant proteins for biotechnological and therapeutic applications at a large scale is an essential need of mankind. With the huge application potential of therapeutic and industrial proteins, there has been increasing demand for effective and efficient bioprocessing strategies. Recent progress around recombinant DNA technologies and bioprocessing strategies has paved the way for efficient production of recombinant proteins. Important factors such as insolubility and cost of production need to be considered for large scale production of these recombinant proteins.
Brief description:
Prof. Tukayi Kudanga holds a PhD in biotechnology and is a professor in the Department of Biotechnology and Food Science at Durban University of Technology, South Africa. He is a recognized authority in laccase applications, particularly in biocatalysis and surface functionalization of lignocellulose materials. His areas of expertise include enzyme biotechnology, biocatalysis, and biomaterials. His current research focuses on enzymatic synthesis of bioactive compounds, enzymatic modification of food biopolymers, and development of green technologies for functionalization of lignocellulose materials. He is also interested in the search for novel biocatalysts, developing new applications and modifying enzymes for relevant biotechnological applications. Prof. Kudanga has extensive experience in teaching and research and has published his works widely. He has been invited to be plenary speaker at international conferences and reviews for several scientific journals.